PSXI-2 Circulating Mirnas as non-Invasive Biomarkers of Chronic Kidney Disease and Calcium Oxalate Uroliths in Felines

Abstract Chronic kidney disease (CKD), characterized by progressive loss of function, is a major cause mortality in cats. Another complication the formation calcium oxalate (CaOx) uroliths bladder and kidneys cats, where inflammation has role its pathogenesis. MicroRNAs (miRNAs) are small non-coding RNAs which bind to target mRNA reduce function. Depending on mRNA, they regulate transcription several pro-inflammatory genes. We assessed expression profiles circulating miRNAs blood, collected at natural end-of-life colony retrospective study, healthy (n=10; 5.9-16 yr), CaOx (n=10, 8.6-13.4 CKD 8.3-15.4 yr). Circulating concentrations creatinine, symmetric dimethylarginine, blood urea nitrogen were significantly greater cats compared with controls, indicating dysfunction. Expression miRNAs, measured qRT-PCR, was analyzed using ΔΔCt method normalized U6. There significant upregulation miR-155 stone formers controls (p< 0.05). increased serum urine human patients nephrolithiasis. can also promote oxalate-induced renal oxidative stress suppressing MGP expression. In humans, negatively correlated cytokines including IL-1β, IL-6, TNF-α, suggesting involvement However, downregulated controls. miR-16 Consistent this, there downregulation both Stage 4 CKD. Further, CaOx, but not CKD, included miR-17, miR-19b, miR-27a, miR-27b, whereas let-7a, miR-15a, miR-16, miR-29b, miR-200a only Taken together, profile may serve as important biomarkers initiation progression dysfunction